HISTOLOGICAL ANALYSIS OF GASTRIC MORPHOLOGY IN EXPERIMENTAL CARBON MONOXIDE INTOXICATION USING HEMATOXYLIN-EOSIN STAINING

Abstract

This article presents a histological analysis of gastric morphological changes during experimental carbon monoxide (CO) intoxication using hematoxylin-eosin (H&E) staining. Carbon monoxide is a highly toxic environmental gas capable of inducing severe tissue hypoxia and structural alterations in internal organs. The study was performed on white laboratory rats exposed to chronic CO intoxication. Histological examination of gastric tissues stained with hematoxylin and eosin revealed progressive pathological changes involving the mucosal epithelium, gastric glands, vascular structures, and stromal components. The most prominent findings included epithelial dystrophy, vascular congestion, edema, inflammatory infiltration, glandular degeneration, and focal necrotic lesions. Hematoxylin-eosin staining proved effective for evaluating both cellular and tissue-level pathological alterations induced by chronic carbon monoxide exposure. The findings demonstrate that prolonged CO intoxication causes significant structural damage to gastric tissues through hypoxia-associated mechanisms.